Flowers are beautiful and quite inspirational as we find them presented out in nature. But there is more hidden within a flower. This image by microphotographer Ray Nelson is actually the base, or ovary, of a flower. Yes, its been enhanced using stain and special lighting, but the pattern and texture is all Mother Nature.
These microphotographs were taken with an American Optical (AO) One Hundred microscope by Leica and a Nikon FA camera. Darkfield Illumination occurs when the focal plane of the aperture is filled precisely by a black stop so that no light is beamed directly through the aperture.
The stoppage creates a black field, while enough light spills around the edges of the stop to bounce from the walls of the objective lens and strike the specimen from oblique angles. This method allows otherwise translucent specimens to be seen clearly and creates in stained specimens a much richer and colorful lighting than would be accessible with brightfield illumination.
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